Pasteurella leukotoxin lktA PCR

Test Schedule
Lab Lab section Days Turnaround
WADDL - Pullman Molecular Diagnostics Mon-Fri 1-10 days
Price: $55.00
Some strains of Mannheimia and Bibersteinia species possess the structural gene for Pasteurella leukotoxin lkta. Since leukotoxin production and secretion may be a virulence factor when conditions are favorable for disease development, identification of strains that possess the lktA gene may be helpful in evaluating the pathogenic potential of these Pasteurellaceae strains carried by wild and domestic animals. Pasteurella multocida is not known to possess the lkta gene, so P. multocida isolates will not be tested for lkta routinely. 

Specimens Required
  • Tracheal or lower respiratory swab (plastic/synthetic swabs preferable. Do not submit swabs in agarose media or calcium alginate swabs, since those inhibit PCR)
  • 1-2mL Transtracheal wash (TTW)  
  • 1 g fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) lung tissue
  • Cultured Pasteurellaceae specimens in broth media or on plated media
  • Primary zones from blood agar culture plates of the inoculated with the samples above, or other agar media selective for Pasteurellaceae, such as CSA.
Sampling Requirements
For additional information on how to collect and submit a sample please visit our Submit a Sample page.
Collection Container
Tissues, swabs, washes: sealed, leak-proof plastic bag or container.
Isolate: Culture plate or tube.
Packing Instructions
Label all samples. Store refrigerated. Ship overnight on cold packs in an insulated container.

For additional information, please visit our Packing and Shipping page.
Special Instructions
Ordering Instructions
For online test ordering please visit our Submit a Sample page for additional information. To submit a specimen without submitting through our online portal please see our Forms page for fillable forms and further instruction.
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