Mycobacterium spp. Identification and sequencing

Test Schedule
Lab Lab section Days Turnaround
WADDL - Pullman Molecular Diagnostics Mon-Fri 5-36 days
Price: $105.00
Detection of Mycobacterium by PCR, with speciation by Sanger sequencing.
Specimens Required
  • 1 g fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissue
  • Aspirates or biopsies in red-top tube
Sampling Requirements
Call lab prior to submission, to understand limitations of this test.
If available, please include histology or cytology reports with submission.

For additional information on how to collect and submit a sample please visit our Submit a Sample page.
Collection Container
Tissues or aspirates: sealed, leak-proof plastic bag or container.
Packing Instructions
Label all samples. Store refrigerated. Ship overnight on cold packs in an insulated container.

For additional information, please visit our Packing and Shipping page.
Special Instructions
WADDL also offers culture for Mycobacterium species, and this is recommended for cases where there is a granulomatous lesion with no acid fast bacteria or few acid fast bacteria seen on a smear or histopathology stain. WADDL can sometimes identify Mycobacterium species when few or no organisms are seen on the stain, but the chances are less than 20% for these cases.

Cultures to rule out M. tuberculosis or requests for specific PCR for the M. tuberculosis group should be sent to NVSL rather than WADDL.

We attempt nested PCR for negative samples in order to improve sensitivity, and we charge a lower fee for nested PCR for samples which yield negative direct PCR results, or for samples which yield poor sequence data. As of February 2023, that fee is $75.
Ordering Instructions
For online test ordering please visit our Submit a Sample page for additional information. To submit a specimen without submitting through our online portal please see our Forms page for fillable forms and further instruction.
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