B. salamandrivorans (Chytrid fungus) PCR

Test Schedule
Lab Lab section Days Turnaround
WADDL - Pullman Molecular Diagnostics Mon-Fri 1-9 days
Price: $62.50
Detection of B. salamandrivorns (Chytrid fungus) by real-time PCR.

Specimens Required
Collecting post-metamorphic amphibian samples to test for Bd or Bsal by PCR:
Swab the animal to collect cells for testing (may pool up to 4 swabs into a tube if desired).
Using synthetic swabs, preferably fine tip (such as Advantage Bundling/Medical Wire Co Catalog #: MW113), gently swab ventral surface of skin 20-30 times.
  • 5 passes of pelvic path
  • 5 passes each side of ventral thighs
  • 5 passes each foot of toe webbing
Cut or break swab so it fits in an empty screw-top tube. Air-dry for ~5 minutes.  Close and label tube containing swab(s). May refrigerate up to 1 week. Freeze if storing longer than 1 week before submitting.
Sampling Requirements
For additional information on how to collect and submit a sample please visit our Submit a Sample page.
Collection Container
Swabs: sealed, leak-proof plastic bag or container.
Packing Instructions
Label all samples. Store refrigerated. Ship overnight on cold packs in an insulated container.

For additional information, please visit our Packing and Shipping page.
Special Instructions
Ordering Instructions
For online test ordering please visit our Submit a Sample page for additional information. To submit a specimen without submitting through our online portal please see our Forms page for fillable forms and further instruction.
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